Two Undescribed Species of Order Hypocreales from The Soil in Korea

Two Undescribed Species of Order Hypocreales from The Soil in Korea

Jun-Woo Choi1, Whee Phaund1,2, Kallol Das1, Kyoung-Taek Park1, Seung-Yeol Lee1*, and Hee-Young Jung1

1College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Korea

2Horticulture and Plant Biotechnology Division, Department of Agriculture, Ministry of Agriculture, Livestock & Irrigation, Nay Pyi Taw, Myanmar

*Email: leesy1123@knu.ac.kr

Two unrecorded species belonging to the order Hypocreales were isolated from soil samples designated as KNU-4-KH1, KNUF-20-NI011 collected from Gyeongbuk province, Korea. The colonies of KNU-4-KH1 initially appeared white, turned pale green on potato dextrose agar (PDA), and the conidia were oblong or ellipsoidal, smooth-walled, greenish, and with a diameter of 2.0–4.4×2.2–3.6 μm. The colonies of KNUF-20-NI011 were yellowish to brown within the center on PDA, and the conidia were ellipsoidal, with a flat hilum, slightly curved with one side slightly flattened, and with a diameter of 6.3–6.8×3.4–3.8 μm. Sequences of internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1-α), β-tubulin (TUB2), and calmodulin (CAL) genes were used to confirm the strains. In case of KNU-4-KH1, the BLAST results indicated the similarities of 100, 98.7, and 99.3% from sequences of ITS regions, TEF1-α, and CAL, respectively, with the strain of Trichoderma ivoriense (CBS 125734) and formed same clade in phylogenetic tree. The strain KNUF-20-NI011, showed similarities of 99.3 and 98.5%, with the strain of Clonostachys divergens (CBS 967.73b) for ITS regions and TUB2, respectively, and closely clustered in phylogenetic tree. Thus, the strains were identified as T. ivoriense (KNU-4-KH1) and C. divergens (KNUF-20-NI011) based on morphological, cultural, and phylogenetic analyses which is the first report in Korea.