Screening of Antagonistic Microorganisms Against Fusarium solani, a Causal Agent of Root Rot of Cnidium officinale Makino

Screening of antagonistic microorganisms against Fusarium solani, a causal agent of root rot of Cnidium officinale Makino

Hyeong Ho Park1, Hee Sang Yun1, Chung Ryul Jung2,*, Hyong Woo Choi1,*

1Department of Plant Medicals, College of Life Sciences and Biotechnology, Andong National University, Andong, Republic of Korea

2Forest Entomology and Pathology, National Institute of Forest Science, Seoul, Republic of Korea

*Email: hwchoi@anu.ac.kr, crjung92@korea.kr

Cnidii Rhizoma (Cnidium officinale Makino) is a plant of the Umbelliferae family, and has been used for a long time to improve blood circulation as an oriental medicine. The biggest problem in cultivating Cnidii Rhizoma is that continuous cropping is impossible due to severe root rot disease. Once it is cultivated, it takes about 5 to 7 years for re-cultivation. Therefore, in this study, we tried to solve the problem occurring during continuous cropping in an environmental-friendly way by developing a biological control agent (BCA) against Fusariumsolani, which is known as the causal agent of root rot of Cnidium Rhizoma. For the screening of BCA, about 300 microorganisms were isolated from the soil and screened by dual culture assay in Potato Dextrose Ager (PDA) media. As a result, 26 microorganisms showed antagonistic activity against F. solani. Among them, 3 bacterial isolates (CAA448-12, BEA1-17, ESP2-15) showing the best antagonistic activity were selected and examined. Especially, ESP2-15 is identified as Brevibacillus spp. via 16s rDNA sequencing and phylogenetic tree analysis. In summary, this study reports candidate strains (CAA448-14, BEA1-17, ESP2-15) which can be useful for controlling root rot of Cnidii Rhizoma in an environmental-friendly way.