New Records of Two Species Belonging to Family Powellomycetaceae from Island in Korea
Dong Hee Kim, Sun Jeong Jeon and Hyang Burm Lee*
Environmental Microbiology Lab, Dept. of Agricultural Biological Chemistry, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 61186, Korea;
Powellomycetaceae is a family of soil-inhabiting chytrids that have a worldwide distribution. Currently, the family has been known to consist of 4 genera and 9 species. However, no members of Powellomycetaceae have been reported in Korea up to now. During a study of chytrid fungal diversity from islands in Korea, two species belonging to family Powellomycetaceae, CNUFC-CHS41-1 and CNUFC-CHS41-2 were isolated from soil samples collected at Jeobdo Island in Korea. Sequence analysis by BLASTn search indicated that the isolates, CNUFC-CNUFC-CHS41-1 and CNUFC-CHS41-2 were closest to Fimicolochytrium alabamae (GenBank accession no. NG_060390) and Powellomyces hirtus (GenBank accession no. JN941005), with identity values of 100 % (900/900 bp), and 100 % (950/950 bp), respectively. Optical microscope observations revealed Sporangium spherical with a single discharge pore, rhizoids, motility zoospore. Based on the morphological characteristics and phylogenetic analyses of large subunit (LSU) and small subunit (SSU), the strains of CNUFC-CHS41-1 and CNUFC-CHS41-2 were identified as Fimicolochytrium alabamae and Powellomyces hirtus, respectively. Herein, their detailed morphological descriptions and phylogenetic trees are provided in this poster.