Isolation and Pure Cultivation of Desarmillaria ectypa and Some Its Properties
Takashi Maruyama1, Takaki Kashiro2, Taiga Kasuya3, Satoshi Takesono2 and Masayuki Onodera1,2*
1Niigata Prefectural Microbiology Society
2Department of Engineering, Niigata Institute of Technology
3Department of Economics, Keio University
Desarmillaria ectypa is one of the rare paludal fungi and is classified as Endangered Category II by the Ministry of the Environment in Japan. The fungus was newly collected from marshy habitats of Joetsu area of Niigata Prefecture at October 2016. The species collected Niigata Prefecture was identified as D. ectypa with supports of morphological observations and phylogenetic analysis. The presence of the species was observed by the naked eyes at the same place at October 2019, 2020, and 2021. So, we collected a minimal amount of the species at November 2022 and attempted isolation and pure cultivation of the species from the spores of fruiting body using Potato Dextrose agar medium containing 50 ppm of ampicillin to prevent bacterial contamination. After one week of static cultivation, germination was observed at 20°C, but no germination was observed at 25°C and 30°C. When the cultivation was continued at 20°C, the formation of rhizomorphs was observed. The mycelia grew between 10°C and 30°C. Addition of yeast extract enhanced the growth of the mycelia using inorganic salt agar medium containing glucose and ammonium sulfate.