Isolation and Characterization of Wild Yeasts from Gut of Batillaria cumingii in Korea

Batillaria cumingii abundantly inhabits the tidal flats with rocks or gravel and the shallow coastal zone of the intertidal. They reproduce by oviparous and are known to be omnivorous. B. cumingii is tolerant of hard intertidal conditions such as temperature variation, salinity, and pollution. In this study, we isolated and characterized the wild yeast strains from the gut of B. cumingii and analyzed their ability to decolorize and detoxify of several xenobiotics. The 9 yeast strains were isolated from the gut of B. cumingii samples from tidal flats in Goha island, Mokpo-si, Jeollanam-do, Korea. Among the yeast strains isolated from the gut of B. cumingii, 7 strains belonged to Ascomycetous yeast, and 2 strains belonged to Basidiomycetous yeast. There is no official report that describes these four yeast species: two strains of the genus Nakaseomyces, one strain of the genus Crinitomyces in the family Saccharomycetaceae, one strain of the genus Metschnikowia in the family Metschnikowiaceae, and one strain of the genus Wickerhamomyces in the family Phaffomycetaceae. Nakaseomyces glabratus D-7 and D-11, Crinitomyces flavificans D-10, Metschnikowia bicuspidata D-13, and Wickerhamomyces anomalus D-14 were recorded in Korea, for the first time.

This work was in part supported by the Honam National Institute of Biological Resources (HNIBR), Ministry of Environment(MOE), Korea(HNIBR202303123)