Isolation and Characterization of Unrecorded Wild Yeast Strains from flowers (Rubus hirsutus Thunb. and Brassica napus L.) on Goha island in Korea

Yeast species are associated with flowers, plants, insects, and nectar and they are common inhabitants of the phyllosphere. In spring, we collected wild flowers (Rubus hirsutus and Brassica napus) from different places and isolated yeast strains on YPD media at 25℃. Twelve yeast strains were isolated from Rubus hirsutus and Brassica napus from in Goha island, Mokpo-si, Jeollanam-do, Korea. To identify wild yeasts, microbiological characteristics were observed by API 20C AUX kit. Pairwise sequence comparisons of D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool (BLAST). Cell morphologies of yeast strains were examined by phase contrast microscope. Among them, 5 strains were already recorded, and seven strains were unrecorded in Korea. Among them, seven strains belonged to Ascomycetous yeast, and five strains belonged to Basidiomycetous yeast. There is no official report that describes these three yeast species: all unrecorded strains of the genus Aureobasidium in the family Saccotheciaceae. Aureobasidium namibiae J-10, Aureobasidium pini Y-10, and Aureobasidium pullulans J-11, J-19, J-4, J-5, and Y-11 were recorded in Korea, for the first time.

This work was in part supported by the Honam National Institute of Biological Resources (HNIBR), Ministry of Environment(MOE), Korea(HNIBR202303123)