Compared to white enoki mushrooms, brown enoki mushrooms are highly adaptable to high temperatures and they are popular among consumers because they have a crispy texture and taste. However, each farm has a different cultivation method and there is a lack of research on cultivation technology that can be used by farmers. Accordingly, this study was conducted to establish a bottle cultivation technology that can produce high-quality mushrooms and increase the quantity by identifying the optimal germination method of brown enoki mushrooms. To assess the growth differences of the brown enoki mushroom ‘Yeoreumhyang1ho’ using various primordium formation methods, the surface of the mycelia in the culture bottle was scratched, and then treated in four different ways as follow. The treatment was performed with pour out the water and place the bottles upside down (T1), place the bottles upside down (T2), pour out the water and place the bottles right side up (T3), place the bottles right side up (T4, control), and the period of each growth stage and the growth characteristics of the fruiting body were investigated. After investigating the growth stages according to the primordium formation methods, it was observed that primordium formation was initiated on the 8th day in the T1 treatment, while in the T4 treatment, it began on the 11th day. The results of the investigation into the characteristics of the fruiting body showed that in T1 and T2, where the bottles were turned upside-down, there was a tendency for smaller pileus diameters, longer stipe lengths, and higher yields compared to the bottles placed right side up. In addition, T1 and T3, treated with irrigation, tended to have fewer valid stipes compared to the untreated. Compared to T4(control), which is currently the most used in farmers, the yield was superior at 181.6g and 178.8g in T1 and T2, respectively, compared to 148.5g in the control. However, in T2, the number of valid stipes increased by 72% to 321 compared to 186 controls, and the pileus diameters was small and the stipe lengths was long.