Effects of Lentinula edodes Water Extract and Lentinan on Myosatellite Cell Proliferation of Bos taurus

Effects of Lentinula edodes Water Extract and Lentinan on Myosatellite Cell Proliferation of Bos taurus

Sohee Kim¹* · Sehyuk Oh² · Sanghun Park² · Eunjin Kim³ · Jungseok Choi² · Hwayong Lee¹

¹Department of Forest Scienece, Chungbuk National University

²Department of Animal Scienece, Chungbuk National University

³Department of Forest Therapy, Chungbuk National University

*Email: ksh1480@chungbuk.ac.kr

In this study, the effects of Lentinula edodes water extracts and lentinan, a beta-glucan, on the proliferation of Bos taurus myosatellite cells were studied. Water extraction conditions were 4 hours at 85 °C, 4 hours at 100 °C, 8 hours at 40 °C, and 24 hours at room temperature, and, lentinan used a commercially available product. L. edodes water extract and lentinan were added to the culture of B. taurus myosatellite cells. Proliferation of the myosatellite cells was confirmed by cell number, cell viability, and expression of MyoD and Myogenin genes. The beta-glucan content of the L. edodes water extract was approximately 15.20% at 85 °C for 4 h, 13.64% at 100 °C for 4 h, 9.48% at 40 °C for 8 h and 8.21% at room temperature for 24 h. The expression of the MyoD gene increased in the addition of the extract at 40 °C for 8 h and 100 °C for 4 h, and the expression of the Myogenin gene increased in the addition of the extract at 40 °C for 8 h, but cell number and cell viability did not increase compared to no addition. However, the addition of lentinan to the culture of B. taurus myosatellite cells increased the expression of the Myogenin gene related to muscle formation increased and the proliferation and viability of the cells. This study proved that the components of L. edodes can affect the proliferation of B. taurus myosatellite cells.