Cordyceps militaris, which belongs to entomopathogenic fungi, is used as medicinal mushroom with diverse activities. It produces a major bioactive constituent called cordycepin, which has various pharmacological effects. Therefore, C. militaris was cultivated using six edible insects(Bombyx mori, Tenebrio molitor, Gryllus bimaculatus, Caelifera sp., Allomyrina dichotoma and Protaetia brevitarsis) and the cordycepin content was analyzed between growth stages of mycelium and fruiting body, under three different temperature conditions(20℃, 25℃, 30℃). The content of cordycepin was high at the 15th day of mycelial growth stage at 30℃, as measured by HPLC analysis. The fruiting-body showed similar patterns with mycelium similar related to temperature conditions, but the content of cordycepin was the highest from the 25th to the 30th day. The cordycepin content was higher in fruiting body than the mycelium. Therefore, it was confirmed that the production of cordycepin varies depending on the type of substrates and cultivation conditions, which can be applied to the characterization of the mechanism of cordycepin production.