Analyses of Transcriptome and MSDIN Genes of 15 Amanita Species from China

The genus Amanita is a famous mushroom group with very important both economical and ecological values. The poisonous mushrooms in the genus Amanita are rich, and 90% of mushroom poisoning incidents are caused by lethal Amanita. To analyze the toxin genes of Amanita species, the transcriptome of 15 Amanita species from China were obtained. The sequencing results showed that the number of unigenes obtained from 15 Amanita species ranged from 28 104 to 66 338. The edible A. ochracea and A. sinensis had the most unigenes, both more than 60 000, and were more twice than those of A. oberwinklara possessing the least unigenes. Seven databases were used for function annotation of unigenes, and The number of unigenes annotated in the NR database is the highest, accounting for 71.30% to 94.19% of the total. Local blast software was used to retrieve the MSDIN genes encoding toxins from the obtained transcriptome data. The results showed that 75 MSDIN gene sequences were obtained from 15 species of Amanita spp., and 55 cyclic peptides composed of 6-10 amino acids were predicted, of which 26 may be new cyclic peptides.